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Scientists offer proposed guidelines for testing the ecotoxicity of microplastics and nanoplastics on living organisms and systems. As concern about microplastics grows, scientists need reliable ways to measure their effects on the environment. This paper presents a standardized protocol for testing the toxicity of microplastics and nanoplastics in soil and aquatic ecosystems. Unlike many existing methods, it uses plastic particles that better reflect those found in the real world and accounts for how they behave in different environmental conditions. By improving consistency across studies, the protocol can help researchers produce more accurate results and strengthen future environmental risk assessments.